Identification of the Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase Gene
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چکیده
منابع مشابه
Identification of the Escherichia coli nicotinic acid mononucleotide adenylyltransferase gene.
The gene (ybeN) coding for nicotinate mononucleotide adenylyltransferase, an NAD(P) biosynthetic enzyme, has been identified and overexpressed in Escherichia coli. This enzyme catalyzes the reversible adenylation of nicotinate mononucleotide and shows product inhibition. The rate of adenylation of nicotinate mononucleotide is at least 20 times faster than the rate of adenylation of nicotinamide...
متن کاملThe Escherichia coli NadR regulator is endowed with nicotinamide mononucleotide adenylyltransferase activity.
The first identification and characterization of a catalytic activity associated with NadR protein is reported. A computer-aided search for sequence similarity revealed the presence in NadR of a 29-residue region highly conserved among known nicotinamide mononucleotide adenylyltransferases. The Escherichia coli nadR gene was cloned into a T7-based vector and overexpressed. In addition to functi...
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The intracellular parasite Trypanosoma cruzi is the aetiological agent of Chagas disease, a public health concern with an increasing incidence rate. This increase is due, among other reasons, to the parasite's drug resistance mechanisms, which require nicotinamide adenine dinucleotide (NAD+). Furthermore, this molecule is involved in metabolic and intracellular signalling processes necessary fo...
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It has now been firmly established that mammals and Neurospora are able to convert tryptophan to nicotinic acid via a biosynthetic pathway which contains several well characterized intermediate compounds (2). However, evidence has been accumulating which indicates that in bacteria the biosynthesis of nicotinic acid is accomplished by a different pathway. Volcani and Snell (3) demonstrated that ...
متن کاملIdentification of Isn1 and Sdt1 as Glucose- and Vitamin-regulated Nicotinamide Mononucleotide and Nicotinic Acid Mononucleotide 5 -Nucleotidases Responsible for Production of Nicotinamide Riboside and Nicotinic Acid Riboside*
Katrina L. Bogan, Charles Evans , Peter Belenky, Peng Song, Charles F. Burant , Robert Kennedy, and Charles Brenner From the Biochemistry Graduate Program, Dartmouth Medical School, Lebanon, New Hampshire 03756, the Department of Biochemistry, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242, and the Department of Chemistry and the Michigan Metabolomics and Obesity Center, ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2000
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.182.15.4372-4374.2000